Abstract and Experimental [2]

Look up abstract and abstraction in Wiktionary, the free dictionary.

Abstract may mean:

• Abstract (law), a brief statement of the most important points of a long legal document or of several related legal papers
• Abstract (summary), an abbreviated summary of any in-depth analysis of a particular subject or discipline
• Abstract art, art that does not depict objects in the natural world
• Abstract class (see Abstract and Concrete classes), a class in object-oriented programming that is designed only as a parent class and from which child classes may be derived, and which is not itself suitable for instantiation
• Abstract hip hop, a subgenre of alternative hip hop distinguished principally on the content of the lyrics
• Abstract music a disambiguation page
• Abstract structure, a set of rules, properties and relationships that is defined independently of any physical objects

Abstraction, a related term, may mean:

• Abstraction, the thought process wherein ideas are distanced from objects
• Abstraction (computer science), a mechanism and practice to reduce and factor out details so that one can focus on few concepts at a time
• Abstraction (mathematics), the process of extracting the underlying essence of a mathematical concept, removing any dependence on real world objects
• Abstraction (sociology), the varying levels at which theoretical concepts can be understood

In the scientific method, an experiment is a set of actions and observations, performed to support or falsify a hypothesis or research concerning phenomena. The experiment is a cornerstone in the empirical approach to knowledge. See the list of famous experiments for historically important scientific experiments.

The word is derived from the Latin ex- + -periri, "from trying".

An experiment in baking

As a simple example, consider that many bakers have noticed that the amount of "fluffiness" in a loaf of bread seems to be related to how much humidity there is in the air when the dough is being made. This can be formalized as the hypothesis: "all other things being considered equal, the greater the humidity, the fluffier the bread".

Whilst this hypothesis might arise naturally from baking many loaves over time, an experiment to determine whether this is really true would be to carefully prepare bread dough, as identically as possible, on two types of days: days when the humidity is high, and days when the humidity is low. If the hypothesis is true, then the bread prepared on the high humidity days should be fluffier.

Several features of this experiment hold in general for all experiments:

• We must try to make all other conditions of the process as similar as possible between the trials. For example, the amounts of flour and water added, the temperature of the butter, and the amount of kneading all may have an effect on the fluffiness; so the experiment should explicitly attempt to control the other variables which could have an effect on the outcome. This gives us some confidence in the statement "all other things being equal,...".

• Although "fluffiness" may seem to be an easily understood idea, one baker's idea of "fluffy bread" may be different than another baker's. The experiment must be based on objective quantities - for example "fluffiness is measured as the total volume of the loaf of bread from one pound of flour". This idea, coupled with the exactness of the description of how the experiment is to be performed, is sometimes called the operational aspect of the experiment; the idea that all actions, quantities, and observations can be agreed upon by reasonable people.

• Noting that once, on a humid day, one baked a fluffy loaf is not enough. The experiment should be repeatable; given that one performs the experiment exactly as described, one should expect to see the same results, no matter who performs the experiment or how many times it is performed.

Repeatability of an experiment helps to eliminate various types of experimental errors - one may think that one has accurately described all of the relevant techniques and measurements in an experiment, but certain other effects (such as the brand of the flour, trace impurities in the water used in the dough, etc.) may actually be contributing to the observed effects. In the scientific method, someone may claim that they have performed an experiment with a particular result, and thereby supported a particular hypothesis. However, until other scientists have performed the same experiment in the same way and gotten the same results, the experiment is usually not considered as a "proven" result (see cold fusion for a recent example).

• Finally, even though one has baked bread a hundred times, occasionally a loaf will completely fail "because the kitchen gods are unhappy". It is important to realize that some hypotheses cannot be tested experimentally - since we cannot make a measurement which will tell us whether or not the "kitchen gods" are "happy", we cannot perform an experiment which either proves or disproves the hypothesis "the best bread happens when the kitchen gods are happy".

Design of experiments

Design of experiments attempts to balance the requirements and limitations of the field of science in which one works so that the experiment can provide the best conclusion about the hypothesis being tested.

In some sciences, such as physics and chemistry, it is relatively easy to meet the requirements that all measurements be made objectively, and that all conditions can be kept controlled across experimental trials. On the other hand, in other cases such as biology, and medicine, it is often hard to ensure that the conditions of an experiment are performed consistently; and in the social sciences, it may even be difficult to determine a method for measuring the outcomes of an experiment in an objective manner.

For this reason, sciences such as physics are often referred to as "hard sciences", while others such as sociology are referred to as "soft sciences"; in an attempt to capture the idea that objective measurements are often far easier in the former, and far more difficult in the latter.

In addition, in the soft sciences, the requirement for a "controlled situation" may actually work against the utility of the hypothesis in a more general situation. When the desire is to test a hypothesis that works "in general", an experiment may have a great deal of internal validity, in the sense that it is valid in a highly controlled situation, while at the same time lack external validity when the results of the experiment are applied to a real world situation. One of the reasons why this may happen is because of the Hawthorne effect.

As a result of these considerations, experimental design in the "hard" sciences tends to focus on the elimination of extraneous effects (type of flour, impurities in the water); while experimental design in the "soft" sciences focuses more on the problems of external validity, often through the use of statistical methods. Occasionally events occur naturally from which scientific evidence can be drawn, which is the basis for natural experiments. In such cases the problem of the scientist is to evaluate the natural "design".

Controlled experiments

Main article: Control experiment

Many hypotheses in sciences such as physics can establish causality by noting that, until some phenomenon occurs, nothing happens; then when the phenomenon occurs, a second phenomenon is observed. But often in science, this situation is difficult to obtain.

For example, in the old joke, someone claims that they are snapping their fingers "to keep the tigers away"; and justifies this behavior by saying "see - its working!" While this "experiment" does not falsify the hypothesis "snapping fingers keeps the tigers away", it does not really support the hypothesis - not snapping your fingers keeps the tigers away as well.

To demonstrate a cause and effect hypothesis, an experiment must often show that, for example, a phenomenon occurs after a certain treatment is given to a subject, and that the phenomenon does not occur in the absence of the treatment. (See Baconian method.)

Standard curve

A controlled experiment generally compares the results obtained from an experimental sample against a control sample, which is practically identical to the experimental sample except for the one aspect whose effect is being tested. In many laboratory experiments it is good practice to have several replicate samples for the test being performed and have both a positive control and a negative control. The results from replicate samples can often be averaged, or if one of the replicates is obviously inconsistent with the results from the other samples, it can be discarded as being the result of an experimental error (some step of the test procedure may have been mistakenly omitted for that sample). Most often, tests are done in duplicate or triplicate. A positive control is a procedure that is very similar to the actual experimental test but which is known from previous experience to give a positive result. A negative control is known to give a negative result. The positive control confirms that the basic conditions of the experiment were able to produce a positive result, even if none of the actual experimental samples produce a positive result. The negative control demonstrates the base-line result obtained when a test does not produce a measurable positive result; often the value of the negative control is treated as a "background" value to be subtracted from the test sample results. Sometimes the positive control takes the form of a standard curve.

An example that is often used in teaching laboratories is a controlled protein assay. Students might be given a fluid sample containing an unknown (to the student) amount of protein. It is their job to correctly perform a controlled experiment in which they determine the concentration of protein in fluid sample (usually called the "unknown sample"). The teaching lab would be equipped with a protein standard solution with a known protein concentration. Students could make several positive control samples containing various dilutions of the protein standard. Negative control samples would contain all of the reagents for the protein assay but no protein. In this example, all samples are performed in duplicate. The assay is a colorimetric assay in which a spectrophotometer can measure the amount of protein in samples by detecting a colored complex formed by the interaction of protein molecules and molecules of an added dye. In the illustration, the results for the diluted test samples can be compared to the results of the standard curve (the blue line in the illustration) in order to determine an estimate of the amount of protein in the unknown sample.

Controlled experiments can be performed when it is difficult to exactly control all the conditions in an experiment. In this case, the experiment begins by creating two or more sample groups that are probabilistically equivalent, which means that measurements of traits should be similar among the groups and that the groups should respond in the same manner if given the same treatment. This equivalency is determined by statistical methods that take into account the amount of variation between individuals and the number of individuals in each group. In fields such as microbiology and chemistry, where there is very little variation between individuals and the group size is easily in the millions, these statistical methods are often bypassed and simply splitting a solution into equal parts is assumed to produce identical sample groups.

Once equivalent groups have been formed, the experimenter tries to treat them identically except for the one variable that he or she wishes to isolate. Human experimentation requires special safeguards against outside variables such as the placebo effect. Such experiments are generally double blind, meaning that neither the volunteer nor the researcher knows which individuals are in the control group or the experimental group until after all of the data has been collected. This ensures that any effects on the volunteer are due to the treatment itself and are not a response to the knowledge that he is being treated.

In human experiments, a subject (person) may be given a stimulus to which he or she should respond. The goal of the experiment is to measure the response to a given stimulus. (Example???)

Natural experiments

Sometimes controlled experiments are prohibitively difficult, so researchers resort to natural experiments. Natural experiments take advantage of predictable natural changes in simple systems to measure the effect of that change on some phenomenon.

Much of astronomy relies on experiments of this type. It is clearly impractical, when trying to prove the hypothesis "suns are collapsed clouds of hydrogen", to start out with a giant cloud of hydrogen, and then perform the experiment of waiting a few billion years for it to form a sun. However, by observing various clouds of hydrogen in various states of collapse, and other implications of the hypothesis (for example, the presence of various spectral emissions from the light of stars), we can collect the experimental data we require to support the hypothesis.

An early example of this type of experiment was the first verification in the 1600s that light does not travel from place to place instantaneously, but instead has a measurable speed. Observation of the appearance of the moons of Jupiter were slightly delayed when Jupiter was farther from Earth, as opposed to when Jupiter was closer to Earth; and this phenomenon was used to demonstrate that the difference in the time of appearance of the moons was consistent with a measurable speed of light.

Quasi-experiments

Quasi-experiments are very much like controlled experiments except that they lack probabilistic equivalency between groups. These types of experiments often arise in the area of medicine where, for ethical reasons, it is not possible to create a truly controlled group. For example, one would not want to deny all forms of treatment for a life-threatening disease from one group of patients to evaluate the effectiveness of another treatment on a different group of patients. Researchers compensate for this with complicated statistical methods. See also quasi-empirical methods.

Examples

• MTT assay
• Colony Formation Assay
• Ames Test
• western blot

Quotes

"We have to learn again that science without contact with experiments is an enterprise which is likely to go completely astray into imaginary conjecture." — Hannes Alfven

"Today's scientists have substituted mathematics for experiments, and they wander off through equation after equation, and eventually build a structure which has no relation to reality." — Nikola Tesla

See also

• Thought experiment
• Significant Figures
• Human experimentation
• Vivisection
• Double-blind
• Self-experimentation
• Experiment in vivo
• Experiment in vitro
• Experiment in silico

External links

• [1] Trochim, William M. The Research Methods Knowledge Base, 2nd Edition. (version current as of January 15, 2005).
• Description of weird experiments (with film clips)

Literature

The Character of Physical Law, by Richard P. Feynman